DEVELOP AND FIELD EVOLUTION OF SINGLE TUBE NESTED PCR, SYBRGREEN PCR METHODS, FOR THE DIAGNOSIS OF LEPROSY IN PARAFFIN-EMBEDDED FORMALIN FIXED TISSUES IN YUNNAN PROVINCE, A HYPER ENDEMIC AREA OF LEPROSY IN CHINA.

BACKGROUND:Detection and pathology analysis of Mycobacterium leprae using skin biopsy tissues are essential for leprosy diagnosis and monitoring response to treatment.Although formalin fixation of patient tissues may not be ideal for molecular studies, biopsy samples are the most accessible material from suspected cases.Therefore, clinical molecula

Full understanding of complex biological interactions frequently requires multi-color detection capability in doing single-molecule fluorescence resonance energy transfer (FRET) experiments.Existing single-molecule three-color FRET techniques, however, suffer from severe photobleaching of Alexa 488, or its alternative dyes, and have been limitedly

Car accidents, a major US public safety issue, demand precise analysis and predictive models for mitigation.This study asks the following question: Can the safest car routes LEGO Ninjago across the US be determined? The paper analyzes historical data to forecast future accidents and calculates the safest route between two locations.The study builds

Abstract This clinical case module is part of a series of online modules being developed for the medical student curriculum in dermatology.This self-directed resource serves as a supplement to traditional lecture and clinical teaching.The purpose of this module is to help students develop a clinical approach to the evaluation and initial management

South Africa was an early leader in multidisciplinary studies of marine resources, particularly with the Benguela Ecology Programme in the 1980s and 1990s and catch records are available HERBATINT FF2 for some species dating back more than a century.Resources data have focused on trends in catches, fishing effort and changes in distribution and abu